Integrated Analysis by GC(RI), GC-MS and 13C NMR of Fortunella japonica Leaf Volatiles Obtained by Hydrodistillation, Microwave- assisted Hydrodistillation and Hydrolate Extraction

Sylvain Sutour (1) , Luro François (2) , Joseph Casanova (1) , Félix Tomi (1)

1 SPE - Sciences pour l'environnement
2 AGAP-Corse - Amélioration Génétique et Adaptation des Plantes méditerranéennes et Tropicales Corse - Antenne Corse

was investigated by combination of chromatographic (GC, CC) and spectroscopic techniques (GC-MS, 13C NMR). F. japonica essential oil and extracts are complex mixtures of 28-60 compounds being mainly oxygenated sesquiterpenes. The EO composition was dominated by germacrene D (14.9% bêta-elemol (9.1%), cis-guai-6-en-10bêta-ol (6.3%),bêta-eudesmol (5.5%), and delta-elemene (5.2%). Limonene was the unique monoterpene identified at appreciable amount (7.1%). The extract obtained by microwave assisted hydrodistillation contained as main components:bêta-elemol (12.4%), germacrene D (9.9%), cis-guai-6-en- 10bêta-ol (9.0%),bêta-eudesmol (8.2%), germacra-1(10),5-dien-4bêta-ol (7.1%) and bêta-eudesmol (6.4%). Finally, the highest content of oxygenated sesquiterpenes (near 92%) was found in the hydrolate extract displaying cryptomeridiol (23.3%, but totally absent in the EO and ME),bêta-eudesmol (20.6%) and alpha-eudesmol (10.7%). Combined analysis by chromatographic and spectroscopic techniques appeared useful for identification of various sesquiterpenols bearing a tertiary alcohol function.

Mots clés: Fortunella japonica; Essential oil; Microwave extract; Hydrolate extract.; Hydrolate extract

Domaines: Chimie analytique

Sylvain Sutour, Luro François, Joseph Casanova, Félix Tomi. Integrated Analysis by GC(RI), GC-MS and 13C NMR of Fortunella japonica Leaf Volatiles Obtained by Hydrodistillation, Microwave- assisted Hydrodistillation and Hydrolate Extraction. Natural Product Communications , 2017, 12 (3), pp.431-434. ⟨10.1177/1934578X1701200331⟩. ⟨hal-02127464⟩