Self-assembled monolayer for AFM measurements of Tobacco Mosaic Virus (TMV) at the atomic level

Matthieu Meillan (1) , Michaël Ramin (1) , Thierry Buffeteau (1) , Sophie Marsaudon (2) , Michaël Odorico (3) , Shu-Wen W. Chen (4) , Jean-Luc Pellequer (5, 6) , Marie Degueil (1) , Karine Heuzé (1) , Luc Vellutini (1) , Bernard Bennetau (1)

1 ISM - Institut des Sciences Moléculaires
2 CBMN - Chimie et Biologie des Membranes et des Nanoobjets
3 SBTN - Service de Biochimie et Toxicologie Nucléaire
4 Vinay
5 IBS - UMR 5075 - Institut de biologie structurale
6 LIRM - Laboratoire des Intéractions et Reconnaissances Moléculaires

Biosensors are based on the conversion of a biological response to an electrical signal. One of the major challenges is to ascertain that the receptor is not denatured when immobilised (covalently or not) on the device. In this work, a protein receptor (virus) was immobilised on two different surfaces, mica and self-assembled monolayer (SAM), and its height was determined by atomic force microscopy measurements at the atomic level. Results clearly showed that expected dimensions of Tobacco Mosaic Virus (TMV) are obtained after immobilisation onto a soft organic SAM.

Domaines: Biologie structurale [q-bio.BM]; Virologie

Matthieu Meillan, Michaël Ramin, Thierry Buffeteau, Sophie Marsaudon, Michaël Odorico, et al.. Self-assembled monolayer for AFM measurements of Tobacco Mosaic Virus (TMV) at the atomic level. RSC Advances, 2014, 4 (23), pp.11927-11930. ⟨10.1039/c3ra46716c⟩. ⟨hal-02010365⟩